Human VDBP ELISA kit (10X96T)
- Known as:
- Human VDBP Enzyme-linked immunosorbent assay test reagent (10X96T)
- Catalog number:
- LF-EK0143
- Product Quantity:
- 10×96T
- Category:
- Peptides
- Supplier:
- Abfron
- Gene target:
- Human VDBP ELISA kit (10X96T)
Related genes to: Human VDBP ELISA kit (10X96T)
- Gene:
- GC NIH gene
- Name:
- GC vitamin D binding protein
- Previous symbol:
- -
- Synonyms:
- DBP, VDBP, hDBP
- Chromosome:
- 4q13.3
- Locus Type:
- gene with protein product
- Date approved:
- 1986-01-01
- Date modifiied:
- 2019-01-25
Related products to: Human VDBP ELISA kit (10X96T)
Related articles to: Human VDBP ELISA kit (10X96T)
- Biodegradation of aviation fuels remains a critical factor during storage and operation, affecting both the composition and performance properties of kerosene fractions. This study aims to establish the relationship between the component composition of modern jet fuels and their biostability. In addition, the effect of typical additives was evaluated. Four industrially used kerosene fractions differing in refining depth were investigated-straight-run, hydrodemercaptanized, hydrotreated, and hydrocracked-along with their mixtures containing antioxidant (Agidol-1) and lubricity additive (Unicor J). Biological tests were conducted under standardized conditions (IP 385/ASTM D6974; GOST 9.023) using Amorphotheca resinae as the test organism; changes in group hydrocarbon composition were analyzed by GC × GC-MS. It was found that susceptibility to biodegradation decreases with increasing refining depth, while microbial activity consistently resulted in a reduction in paraffinic and naphthenic hydrocarbons and a relative increase in aromatics; simultaneously, sulfur content decreases. Paradoxically, the most pronounced increases in acidity and existent gum content were observed in the hydrotreated and hydrocracked fractions. Agidol-1 exhibited biostatic (antimicrobial) activity, but was accompanied by a marked drop in aqueous phase pH (≈ 4), suggesting increased corrosion risk associated with the aqueous phase. Unicor J produced a slight positive effect, likely due to its surface-active properties. - Source: PubMed
Publication date: 2026/08/16
Sharin Evgeny AKarpov Valeriy ASemenova Tatiana AMatveeva Oxana AKrylov Vadym GMakhova Uliana AIlin Andrei VKlimov Nikita ALobashova Marina MKapustin Vladimir MErshov Mikhail A - Acne vulgaris is a multifactorial inflammatory disorder of the pilosebaceous unit characterised by excessive sebum production, follicular hyperkeratinisation, microbial dysbiosis, and inflammatory activation. Limitations of conventional therapies, including irritation and antimicrobial resistance, have increased interest in plant-derived topical agents offering multi-target efficacy with improved tolerability. Spach is traditionally used for dermatological conditions; however, its standardised formulation and mechanistic anti-acne evaluation remain limited. Notably, no previous study has integrated phytochemical characterisation, topical formulation development, mechanistic lipase enzyme evaluation, in vivo sebum suppression, and dermal safety assessment for this species within a single preclinical framework. - Source: PubMed
Publication date: 2026/08/11
Kaweesa Simon PeterNtulume IbrahimLukwago Tony WotoyitideOdoki MartinAnokbonggo Washington WillyGuyah BernardOmbaka James H - Translating CRISPR-Cas9-based homology-directed repair (HDR) strategies into clinical application remains a major challenge due to limited standardization, concerns over safety, and efficacy issues. Here, we present a comprehensive and clinically compliant preclinical framework for the correction of Wiskott-Aldrich syndrome (WAS) using a CRISPR-Cas9-AAV6 platform targeting hematopoietic stem and progenitor cells (HSPCs). In this study, we established a clinical-compatible platform enabling large-scale manufacturing while preserving HSPC viability, stemness, and multilineage functionality. To overcome low HSPC long-term engraftment, we fine-tuned AAV dosing and transiently modulated p53BP-dependent DNA damage response pathway, achieving significantly improved correction and repopulation. Importantly, we implemented a multi-tiered genotoxicity assessment strategy, integrating in silico, genome-wide, and orthogonal assays, revealing a largely favorable safety profile with minimal off-target risks and no evidence of clonal dominance or transformation during the study period. Longitudinal safety monitoring revealed donor-specific rare off-target events and structural variants. This highlights the crucial importance of patient monitoring after transplantation, further emphasized by the identification of a chromosomal rearrangement that could be detected exclusively following cell engraftment in mice. This work offers a robust and adaptable roadmap for future HDR-based gene editing platforms, establishing critical benchmarks for efficacy, safety, and regulatory readiness in the development of advanced therapeutic medicinal products. - Source: PubMed
Publication date: 2026/07/22
Naseem AsmaVetharoy WinstonWhittaker Thomas EZinghirino FedericaCarusillo AntonioGhosh RoopkathaGomez-Castaneda EduardoAli HashimCipriani CarloMontini EugenioThrasher Adrian JSantilli GiorgiaCesana DanielaTurchiano GiandomenicoCavazza Alessia - Metabolic dysfunction associated steatotic liver disease (MASLD) is one of the most predominant liver disorders. MASLD is associated with dietary intake of fatty acids. Among them, oleic (OA) and palmitic (PA) acids are the most abundant. Furthermore, OA was reported to have an hepatoprotective effect against PA toxicity on primary hepatocytes and liver cell lines. In this work, to characterize the mechanisms related to fatty acids exposure, we analyzed the metabolomic signatures of HepG2/C3A using advanced organ-on-chip technology. The cells were exposed for 2 to 7 days to OA, PA and OA/PA mixture. The cell secretions were analyzed using GC-MS spectrometry to reveal their metabolomic signatures. We were able to identify a common HepG2/C3A response to OA, PA and OA/PA deciphering the FFAs global perturbations including the nitrogen metabolism, after 7 days of treatments. Then, we extracted the specificity of each treatment. We found that OA altered intracellular lipid secretion, while OA/PA shifted metabolism from the lipid production to their consumption. PA exposure induced changes consistent with a perturbation in oxidative stress, energy metabolism and a high oxalic acid production. Nicotinamide levels increased in all treatments, indicating a potential exposure biomarker of FFA, and a potential mechanistic candidate for further validation in preclinical and clinical settings as its protective effect against liver steatosis was reported in literature. These findings highlight the intricate metabolic responses to FFAs exposure in hepatocytes, suggesting potential biomarkers and pathways for liver-related disorders. - Source: PubMed
Publication date: 2026/07/30
Morisseau LisaGilard FrançoiseGakière BertrandLegallais CécileSakai YasuyukiLeclerc EricJellali Rachid - The aim of this study was to describe and compare the fatty acid (FA) profiles of hepatic tissue in farmed and wild channel catfish (, Rafinesque, 1818). Thirty-four liver samples were collected from farmed and wild channel catfish. After FA samples were extracted, their fatty acid profiles were obtained by gas chromatography with a flame ionization detector (GC-FID). The analysis revealed that monounsaturated FAs were present at higher concentrations in farmed organisms (161.45 mg/100 g) than in wild organisms (46.87 mg/100 g). While the most abundant FA group in wild organisms was saturated FAs (58.48 mg/100 g), farmed catfish had higher levels (73.22 mg/100 g). The farmed organisms also presented a higher total concentration of FA (296.03 mg/100 g), which is double the mean obtained in wild channel catfish (131.89 mg/100 g). Nonsignificant differences were found in the polyunsaturated, omega-3 and omega-6 quantities, yet a suggestive difference was found for EPA. Our findings suggest that the fatty acid content in the liver is notably different between wild and farm origins. The differences identified in this study were attributed to differential feeding patterns in the analyzed populations of farmed and wild channel catfish. - Source: PubMed
Publication date: 2025/12/20
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