GATA3 (N_term)
- Known as:
- GATA3 (N_term)
- Catalog number:
- EB05570
- Product Quantity:
- 0.1 mg
- Category:
- -
- Supplier:
- ACR
- Gene target:
- GATA3 (N_term)
Related genes to: GATA3 (N_term)
- Gene:
- GATA3 NIH gene
- Name:
- GATA binding protein 3
- Previous symbol:
- -
- Synonyms:
- HDR
- Chromosome:
- 10p14
- Locus Type:
- gene with protein product
- Date approved:
- 1992-11-03
- Date modifiied:
- 2016-10-05
Related products to: GATA3 (N_term)
Related articles to: GATA3 (N_term)
- Mouse mammary epithelial cells possess a remarkable ability to regenerate the entire mammary gland through precisely regulated differentiation, involving complex molecular, morphological, and functional changes. Here, we performed comprehensive transcriptomic profiling of HC11 mouse mammary epithelial cells undergoing lactogenic differentiation using RNA sequencing and integrative bioinformatics. We identified 566 differentially expressed genes, reflecting extensive transcriptional reprogramming and activation of biosynthetic, metabolic, and secretory programs. Strong up-regulation of terminal and lactogenic differentiation markers, including Wap, Csn2, Lpl, Cd36, Lalba, Btn1a1, Xdh, Gata3, and Cebpb, signified maturation into a secretory phenotype. Functional evaluation via gene set enrichment analysis revealed transcriptional enrichment of mTOR, prolactin, insulin, ErbB, and autophagy-associated pathways, consistent with anabolic readiness and terminal differentiation. Conversely, p53, Wnt, and FoxO pathways were down-regulated, marking a transition from proliferation to differentiation. Transcription factors (FoxO1, Zbtb16, and Srebf1) and epigenetic regulators (Gadd45a and Hist1h1e) exhibited dynamic changes, underscoring coordinated transcriptional and chromatin remodeling. Gene set enrichment and protein-protein interaction analyses identified 10 hub genes, Agt, Ccnd1, Igf1, Mki67, Myc, Calm4, Rasgrp1, Cd69, Il6, and Pecam1, as central drivers of differentiation. Clustering of uniquely regulated genes further implicated roles in milk synthesis, protease activity, and lineage stabilization. Together, these findings define a transcriptional framework for lactogenic differentiation in the HC11 cell line model and provide a basis for future mechanistic studies. - Source: PubMed
Publication date: 2026/05/04
Ahmad WaqarPanicker Neena GopinathanRizvi Tahir AMustafa Farah - Somatic mutations in protein-coding genes and noncoding regulatory regions are the major drivers of cancer. Only a relatively small number of somatic noncoding mutations that are likely drivers have been described to date, including those in the promoters of the , and genes. The impact of these alterations can be profound by initiating, increasing, or abolishing gene expression. Promoter mutations in particular have been difficult to identify even from whole tumor genomes due to their high content of G and C nucleotides, which leads to loss of sequencing coverage in these regions. Therefore, the landscape of somatic drivers in gene promoters remains incomplete. Here, we present a hybrid capture assay optimized for >3000 promoters of cancer genes. We show that this assay allows for deep sequencing of challenging GC-rich promoter regions, enabling discovery of reliable point mutations, short insertions and deletions, copy number variants, and mutational signatures in cell line models as well as formalin-fixed, paraffin-embedded archival tissue samples. Our assay nominated candidate noncoding driver mutations in , and in breast cancer for future functional follow-up. - Source: PubMed
Publication date: 2026/05/04
Qi MeifangDave Preshita SanjayFrancis NicoleDeFelice MatthewPollock SamCorbitt HollyMitsiades Irene RinWang GengchaoFrere PaulFox AlanKhitrov GregoryPetersen CarolineLarkin KatieHamilton SusannaEllisen Leif WGulhan Doga CHidalgo-Miranda AlfredoLennon NiallCibulskis CarrieRheinbay Esther - The histopathologic distinction between patch-stage mycosis fungoides (MF) and benign inflammatory dermatoses (BID) remains a persistent diagnostic challenge, often due to overlapping clinical and immunophenotypic features. GATA binding protein 3 (GATA3), a transcription factor critical in T-helper 2 cell differentiation, has emerged as a potential immunohistochemical marker in T-cell neoplasms. This study aimed to evaluate GATA3 expression in patch-stage MF compared with BID to assess its diagnostic value. Sixty formalin-fixed, paraffin-embedded skin biopsies were retrospectively analyzed, including 30 cases of patch-stage MF and 30 cases of BID (psoriasis, chronic dermatitis, and lichen planus). Immunohistochemical staining for GATA3 was performed, and lymphocytic nuclear staining was assessed in dermal and epidermal compartments. GATA3 expression > 50% in dermal lymphocytes was observed in 20% of MF cases and 6.7% of BID cases, yielding high specificity (93.3%) but low sensitivity (20%) for MF diagnosis. Epidermal GATA3 expression was uniformly low across both groups. No significant correlations were found between GATA3 expression and key histopathologic or immunophenotypic features of MF. Although elevated dermal GATA3 expression may support the diagnosis of MF in some cases, its substantial overlap with BID and low sensitivity limit its utility as a reliable standalone diagnostic marker for early-stage MF. GATA3 can be incorporated into broader immunohistochemical panels alongside more specific markers to improve diagnostic accuracy. - Source: PubMed
Publication date: 2026/05/04
Montazer FatemehMalekan MohammadAbedi Hamid - Leucine-rich pentatricopeptide repeat-containing protein (LRPPRC) is a recently identified N6-methyladenosine (m6A) reader protein involved in a myriad of biological processes in cancer. However, its roles in glioma have not been reported. Both in vitro and in vivo studies were performed to examine the anti-glioma activity of LRPPRC silencing. Chromatin immunoprecipitation assay and RNA immunoprecipitation assay were used for the analysis of interaction between upstream and downstream molecules. Methylated RNA immunoprecipitation assay was conducted to examine m6A modification. Our results showed that LRPPRC expression was dramatically upregulated in glioma tissues and cell lines. LRPPRC regulated the m6A modification of protocadherin-7 (PCDH7) and affected its expression in glioma cell lines; LRPPRC silencing significantly inhibited glycolysis and induced ferroptosis in glioma cell lines; this was reversed by PCDH7 overexpression. Furthermore, GATA-binding protein 3 (GATA3) directly bound to the LRPPRC promoter and transcriptionally regulated LRPPRC. LRPPRC mediated the regulatory effects of GATA3 on glycolysis and ferroptosis in glioma cell lines. In vivo studies showed that LRPPRC silencing suppressed tumor growth. Taken together, our data demonstrate that LRPPRC expression was dramatically upregulated in gliomas and that inhibition of the GATA3/LRPPRC/PCDH7 axis exerted an anti-glioma effect by regulating glycolysis and ferroptosis. - Source: PubMed
Publication date: 2026/05/04
Sun YongJia YunlongBu XingyaoChen YushengWang Ruixing - Allergic rhinitis (AR) is a common chronic nasal mucosal inflammatory disorder driven by type 2 immunity, but the spatial stromal-immune cell interactions underlying its pathogenesis remain unclear. - Source: PubMed
Publication date: 2026/04/17
Zhao MiaoDuan JiaqiXie YongminHuang GuanYang GuiYang PingchangZeng Haotao