SOX9
- Known as:
- SOX9
- Catalog number:
- AP15790PU-S
- Product Quantity:
- 0.1 ml
- Category:
- -
- Supplier:
- ACR
- Gene target:
- SOX9
Related genes to: SOX9
- Gene:
- SOX9 NIH gene
- Name:
- SRY-box 9
- Previous symbol:
- CMD1, CMPD1
- Synonyms:
- SRA1
- Chromosome:
- 17q24.3
- Locus Type:
- gene with protein product
- Date approved:
- 1992-09-25
- Date modifiied:
- 2018-06-25
Related products to: SOX9
Related articles to: SOX9
- Introduction Anterior cruciate ligament (ACL)-derived cells have emerged as a promising autologous cell source for ligament tissue engineering and graft augmentation. However, although these cells are commonly expanded in vitro prior to application, their passage-dependent biological changes have not been systematically characterized, particularly in cells obtained during the acute phase after injury. This lack of knowledge may affect the reproducibility and therapeutic reliability of ACL-derived cell-based strategies. This study investigated passage-dependent changes in human anterior cruciate ligament (ACL)-derived cells obtained during the acute phase after injury, aiming to identify the passage range that retains optimal biological properties for ligament tissue engineering and graft augmentation. Methods ACL remnants from 7 patients were collected within 1 month after injury. Cells were isolated by type I collagenase and expanded to passage 5. Cells were isolated by type I collagenase and expanded to passage 5. Morphology was evaluated using optical microscopy and Giemsa staining. Growth kinetics were assessed by doubling time and acumulative cell counts. ECM- and lineage-related genes (COL1A1, COL2A1, COL3A1, COMP, SOX9, TNC, α-SMA) were quantified by RT-qPCR. Results ACL-derived cells preserved fibroblast-like morphology up to passage 3, while passages 4-5 displayed enlarged spacing and rounding. Doubling time decreased after passage 1. Collagen gene expression (COL1A1, COL2A1, COL3A1) declined markedly from passages 3-4 (P < 0.001), while COMP, SOX9, and TNC showed no significant changes. α-SMA also decreased from passage 2. Collagen gene ratios were unchanged despite reduced absolute expression. Conclusions ACL-derived cells retain favorable morphology, proliferation, and ECM-producing capacity within passages 1-3, whereas passages ≥ 4 exhibit observable morphological changes and a possible decline in ligamentogenic capacity. These findings indicate that maintaining expansion within early passages may be preferable for translational applications such as scaffold reseeding and biologically enhanced graft ligamentization. - Source: PubMed
Publication date: 2026/07/28
Park JinsungKim Tae-HwanLee Jin Kyu - Articular cartilage, a unique avascular and low-cell-density connective tissue, relies predominantly on chondrocyte responses to mechanical cues for the maintenance of tissue homeostasis and functional repair. Among the diverse mechanical stimuli encountered in the joint microenvironment, compressive stress stands as the most prominent and physiologically relevant physical signal regulating chondrocyte behavior. This review systematically dissects the multi-layered mechanisms underlying compressive stress-mediated chondrocyte regulation and its translational implications in cartilage tissue engineering and osteoarthritis (OA) intervention. At the molecular level, compressive stress initiates a cascade of mechanosensing, intracellular transduction, and functional output through the synergistic crosstalk of integrin-mediated adhesion complexes, calcium signaling networks, MAPK pathways, and downstream transcriptional regulators (e.g., SOX9, Runx2, Sp1), which collectively orchestrate the balance between anabolic and catabolic metabolism. At the cellular level, articular cartilage's inherent regional heterogeneity, coupled with distinct responses of healthy/pathological chondrocytes and stem cells to compressive parameters (frequency, strain magnitude, loading mode, duration), underscores the need for cell-type-specific mechanical intervention strategies. At the translational level, moderate dynamic compression promotes cartilage repair by preserving extracellular matrix integrity, suppressing inflammatory cascades, and modulating epigenetic landscapes, while aberrant loading exacerbates OA progression via chondrocyte apoptosis, matrix degradation, and pain sensitization. The optimization of scaffold materials (natural polymers, synthetic composites, intelligent responsive matrices) and culture systems (3D bioprinting, microfluidic bioreactors, shear-compression synergistic loading) has emerged as a critical enabler to enhance mechanical regulation efficacy. Despite significant advances, current research is constrained by insufficiently physiological in vitro/in vivo models, lack of standardized loading parameters, unclear pathway crosstalk mechanisms, and limited clinical translation of mechanical-based therapies. Future endeavors should prioritize the elucidation of multi-pathway synergistic networks using multi-omics approaches, establishment of personalized mechanical parameter databases integrating patient-specific factors (age, gender, disease severity), construction of bionic models recapitulating the joint's dynamic microenvironment, and development of combined mechanical-biological therapeutic strategies. These efforts will provide more precise molecular targets and clinically feasible schemes for cartilage repair and OA management. - Source: PubMed
Publication date: 2026/07/14
Mou YongbingTian TingtingWang PengWang XiaLi WeiTang WenfeiWang YehongZhu DongHuang YongHuang Xiao - Brain cortical development results from the proliferation, differentiation, migration and maturation of many cell types. While neuronal development is well characterized, the mechanisms regulating macroglial cells (oligodendrocytes and astrocytes) development remain largely unknown. Recent works suggest that the vascular system plays a key, yet underevaluated role in this process. In this study, we investigated the spatial organization of macroglial cells within the parenchyma and relative to blood vessels. Using immunolabelling for Sry-box transcription factor (Sox) 9 (macroglial progenitors and astrocytes) and Sox10 (oligodendrocyte lineage), we determined macroglia density, distribution and proximity to blood vessels from postnatal day (P) 1 to P60 in the somatosensory cortex. We showed that Sox9+ cells were evenly distributed across cortical layers with regular intercellular spacing. In contrast, Sox10+ cells concentrated in deeper cortical layers and exhibited a random distribution. Vascular density and branching increased markedly between P5 and P15, and macroglial cells were closer to blood vessels from P15 onward. We investigated possible alteration of astrocyte distribution in the cortex of MLC1-deficient mice, a model of megalencephalic leukoencephalopathy with subcortical cysts, in which astrocyte perivascular coverage is altered. No difference with the control condition was found both in young and adult mice, either in the density, distribution or distance to blood vessels. Altogether, we revealed distinct distribution and postnatal development patterns for astrocytes and oligodendrocytes in the brain and in relation to the vasculature. - Source: PubMed
Guille NaomieMonnet HéloïseHourcade TristanMailly PhilippeCohen-Salmon MartineBoulay Anne-Cécile - Boar sperm freezability (SF) is an economically important trait that influences reproductive efficiency and genetic improvement in pigs. However, its genetic basis remains poorly understood. In this study, semen samples from 382 Duroc and 151 Yorkshire boars were evaluated for sperm motility and recovery rate, and all individuals were genotyped using an 80K SNP array. Within-breed GWAS were first conducted, followed by a meta-analysis integrating the GWAS results from both boars. Individuals were classified into GSF and PSF groups based on sperm recovery rate for subsequent selection signature analysis. The results showed that Duroc boars exhibited significantly higher SF than Yorkshire boars. Heritability estimates for SF were moderate, with values of 0.35 in Duroc and 0.30 in Yorkshire. GWAS identified 10 significant SNPs in Duroc and 33 in Yorkshire associated with SF. Meta-analysis further detected 12 significant SNPs, annotated to candidate genes such as CCDC181, PARN, SOX9, and NCKAP5L. Association analysis identified ten representative variants significantly correlated with sperm recovery rate, with variants in PARN and MAP2K6 showing strong additive effects. Selection signature analysis revealed multiple genomic regions under differential selection between GSF and PSF groups, identifying several candidate genes associated with SF. Functional enrichment analysis indicated that these genes are mainly involved in spermatogenesis, flagellar motility, cellular stress response, and cold adaptation pathways. Overall, this study provides novel insights into the genetic architecture of boar SF and identifies potential molecular markers for genetic improvement and functional genomic studies in pigs. - Source: PubMed
Publication date: 2026/07/22
Wu SiwenHe JianLiang QianxiLi XuehuaLu ZhuodaLi ZhiliJi HuiFeng YaoZhuang ZhanweiZhao Yunxiang - Cytogenomics, including karyotyping, FISH, chromosomal microarrays, and optical genome mapping, has yielded significant results for clinical phenotypes in constitutional and cancer genetics, including intellectual disability, autism spectrum disorders, dysmorphic features, and hematological and solid-tissue neoplasia. However, some of these assays have yielded results of unclear significance because the abnormalities detected were often located in intergenic regions of the genome. Because these abnormalities are within the "dark matter" of the genome, their clinical significance has been a matter of speculation. However, functional genomics can explore the clinical implications of such abnormalities more robustly, whether the abnormalities disrupt topologically associating domains (TADs), delete regulatory regions, etc. Some human genetic diseases associated with these intergenic abnormalities and characterized by functional genomics include preaxial polydactyly ( gene), Pierre Robin syndrome (), and 5q14.3 microdeletion syndrome (). While functional genomics is a broad research topic, this review focuses on prior and current efforts to leverage functional genomics within the intergenic regions for human health. - Source: PubMed
Publication date: 2026/06/30
Gonzales Patrick R