SOX9
- Known as:
- SOX9
- Catalog number:
- AP15790PU-N
- Product Quantity:
- 1.0 ml
- Category:
- -
- Supplier:
- ACR
- Gene target:
- SOX9
Related genes to: SOX9
- Gene:
- SOX9 NIH gene
- Name:
- SRY-box 9
- Previous symbol:
- CMD1, CMPD1
- Synonyms:
- SRA1
- Chromosome:
- 17q24.3
- Locus Type:
- gene with protein product
- Date approved:
- 1992-09-25
- Date modifiied:
- 2018-06-25
Related products to: SOX9
Related articles to: SOX9
- Gene therapy combined with advanced biomaterial-based delivery systems represents a powerful strategy to enhance chondrogenic differentiation of mesenchymal stem cells (MSCs), enabling the development of next-generation regenerative therapies for cartilage repair. In this context, gene-activated biomaterials provide a versatile tool for spatially and temporally regulating cell fate within three-dimensional (3D) microenvironments. Here, we combine collagen type I/type II-hyaluronic acid (CI/CII-HyA) scaffold with a novel non-viral gene delivery platform based on niosomes (DP20CQ) to deliver the master chondrogenic transcription factor SOX9 using either parental (PP) or minicircle (MC) plasmids, thereby promoting chondrogenesis in MSCs. After 28 days under chondrogenic conditions, DP20CQ-based scaffolds promoted a more favourable chondrogenic-to-hypertrophic profile than gene-free or Lipofectamine (LPF)-based scaffolds while preserving metabolic activity. Sustained SOX9 overexpression was evidenced in both PP and MC niosome-based systems at the gene and protein levels. Similarly, both systems showed an upregulation of key chondrogenic markers, including aggrecan (ACAN) and collagen type II (COLII), together with the concomitant downregulation of fibrocartilage (collagen type I, COLI) and hypertrophic (collagen type X, COLX) markers, with DP20CQ/MC exhibiting the highest expression ratios. Taken together, these findings demonstrate that DP20CQ-activated biomaterials enable efficient and sustained genetic regulation in MSCs within a 3D microenvironment, promoting the formation of hyaline-like cartilage while suppressing hypertrophic differentiation. This strategy constitutes a versatile gene-activated biomaterial platform with promising potential for cartilage regeneration. - Source: PubMed
Publication date: 2026/06/11
López-Seijas JunqueraIglesias-Fente AlbaIntini ClaudioDobricic MarkoO'Brien Fergal JRey-Rico Ana - Circular RNAs (circRNAs) are important regulators of signaling pathways involved in intervertebral disc degeneration (IVDD). This study investigated the role and underlying mechanism of circTMEM230 in the degeneration of endplate chondrocytes. We observed that circTMEM230 expression was significantly downregulated in chondrocytes subjected to intermittent cyclic mechanical tension (ICMT). Functional assays demonstrated that overexpression of circTMEM230 enhanced the expression of extracellular matrix (ECM)-related genes through modulation of the miR-223-3p/FOXO3/SOX9 signaling axis. Specifically, circTMEM230 acted as a molecular sponge for miR-223-3p, thereby upregulating FOXO3, which subsequently promoted SOX9 transcription. In vivo experiments further confirmed that circTMEM230 mitigated IVDD progression and regulated the expression of miR-223-3p, FOXO3, and SOX9. Additionally, expression levels of circTMEM230, miR-223-3p, FOXO3, and SOX9 were found to be correlated in endplate cartilage tissue samples from IVDD patients. These findings suggest that circTMEM230 exerts a protective role in IVDD and may serve as a promising therapeutic target for further investigation. - Source: PubMed
Zheng QuanYang JiongLi Xing-XingShao SongWang Chuan-DongWang Qi-WeiSun Liang-Ye - The complex microenvironment of articular cartilage defects, characterized by oxidative stress and infection risk, poses a major challenge for regenerative medicine. Here we report a molecular engineering strategy to transform mung bean protein (MBP), an abundant and sustainable plant protein, into a multifunctional, microenvironment-relevant bioink. By sequentially grafting ε-polylysine (EPL) and methacryloyl groups onto the MBP backbone, we created a family of photocurable derivatives (MEM) with tunable methacrylation degrees. Three variants were obtained: MEM-A (65% methacrylation), MEM-B (78% methacrylation), and MEM-C (86% methacrylation). The hydrogels exhibit rapid rheological gelation (<2 s, defined by the '-″ crossover), tunable mechanical properties (20-60 kPa compressive modulus), and a porous architecture (100-150 μm pore size, 70-80% porosity) conducive to cell growth. The resulting MEM hydrogels integrate broad-spectrum antimicrobial activity (>90% bacterial killing), enhanced antioxidant capacity (>51% radical scavenging), and excellent DLP printability (>87% printing accuracy). Among these, the MEM-B hydrogel supports chondrocyte viability, upregulates cartilage-specific genes (), and downregulates oxidative stress markers (). In a rat osteochondral defect model, MEM-B promoted robust cartilage regeneration with superior ICRS scores and tissue integration compared to GelMA controls. This work establishes a design paradigm for converting underutilized plant proteins into multifunctional bioinks that actively engage with pathological microenvironments, opening sustainable avenues for next-generation regenerative biomaterials. - Source: PubMed
Publication date: 2026/06/13
He XiaoliZhou ZhengZhu ShuaiChen XinYan ShipingYang LingxiuLei JiajieDai YaoLiu Hairong - Astrocytes play essential roles in neuronal development, function, and disease, yet existing methods to derive astrocytes from human pluripotent stem cells (hPSCs) are complex and can involve months of maturation. We developed a genomic safe-harbor knock-in system for inducible expression of the astrogenic transcription factors NFIA, NFIB, and SOX9, enabling rapid and robust generation of functional induced astrocytes (iAstrocytes). Across five hPSC lines, NFIB-SOX9 and NFIA-NFIB-SOX9 combinations efficiently generated highly pure populations expressing astrocyte-specific and synaptogenic genes. iAstrocytes displayed cytokine-induced expression of complement factors C3 and C4 and were amenable to CRISPR interference (CRISPRi) gene expression knockdown. Optimization of culture conditions enabled survival of NFIB-SOX9 iAstrocytes in co-culture with human induced neurons (iNeurons). Through pharmacological and genetic perturbations, we uncovered a previously undescribed phenomenon in which co-culture with iAstrocytes promoted the development of synchronized iNeuron network calcium activity mediated by specific gap junction proteins. This rapid and genetically tractable iAstrocyte platform provides a robust model to dissect human genetic and environmental effects on astrocyte-neuron interactions. - Source: PubMed
Publication date: 2026/06/05
Morshed NaderDemers MatthewGonzalez-Ramos AnaJäntti HennaDoman JordanD'Souza SeanLi LetianGranger Adam JJohnson Matthew BStevens Beth - The zig-zag eel () exhibits sexual dimorphism in growth patterns. Identifying the genes involved in sex differentiation is a crucial step toward achieving single-sex breeding and serves as a vital foundation for elucidating the XY sex determination mechanism in . This study measured the morphological characteristics of male and female and found that males were significantly superior to females in body weight and nearly all morphological indices. Subsequently, whole-transcriptome sequencing was performed on the gonads of adult males and females, identifying 11,714 DEmRNAs, 3442 DElncRNAs, 416 DEcircRNAs, and 620 DEmiRNAs, including male sex differentiation genes such as , , , and , and female sex differentiation genes like , , and . Functional enrichment analysis identified pathways associated with sex differentiation, including the TGF-beta signaling pathway, the steroid hormone biosynthesis, the Hippo signaling pathway, and the Wnt signaling pathway, etc. A ceRNA network was constructed based on differentially expressed mRNAs and ncRNAs, revealing that the sex differentiation-related genes , , , , and are regulated by one or multiple pairs of lncRNA/circRNA-miRNA pairs. The study results will provide molecular targets for research on sex-controlled breeding in and lay an important theoretical foundation for clarifying its sex differentiation mechanisms. - Source: PubMed
Publication date: 2026/06/05
Zhu JunxianJia XianghuiJi LiqinChen ChenGao CaixiaHong XiaoyouLiu XiaoliWei ChengqingZhu XinpingLi Wei