Monkey GM-CSF ELISPOT kit, silver staining
- Known as:
- Monkey GM-CSF ELISPOT reagent, silver staining
- Catalog number:
- ct124-pb2
- Product Quantity:
- EUR
- Category:
- -
- Supplier:
- U-CyTech biosciences
- Gene target:
- Monkey GM-CSF ELISPOT kit silver staining
Related genes to: Monkey GM-CSF ELISPOT kit, silver staining
- Gene:
- CSF2 NIH gene
- Name:
- colony stimulating factor 2
- Previous symbol:
- -
- Synonyms:
- GM-CSF, GMCSF
- Chromosome:
- 5q31.1
- Locus Type:
- gene with protein product
- Date approved:
- 2001-06-22
- Date modifiied:
- 2018-12-12
Related products to: Monkey GM-CSF ELISPOT kit, silver staining
Related articles to: Monkey GM-CSF ELISPOT kit, silver staining
- Urinary tract infections (UTIs) are one of the most commonly encountered infections in clinical practice, in which psychological stress is a critical pathological contributor to modulate immune function. However, mechanistic pathways linking stress networks in the brain to bladder infection remain poorly understood. In this study, we discovered that acute stress treatment suppressed bladder inflammation in mice with UTIs, and a significant number of neurons showing overlap between inflammation-associated markers and retrograde labeling were observed in the paraventricular nucleus (PVN) brain region of these mice. Activation of PVN alleviated UPEC-induced bladder inflammatory response. Moreover, blocked hypothalamic-pituitary-adrenal (HPA) axis reversed the anti-inflammatory reflex mediated by acute stress, suggesting that the potential of glucocorticoids levels through the brain-body circuits to ameliorate UTIs. Single cell-RNAseq of bladder immune cells revealed that type 2 innate lymphoid cells (ILC2) expressed abundant levels of glucocorticoid receptor (GR). The activation of PVN effectively inhibited the expression of the proinflammatory cytokine Csf2 by ILC2 through direct regulation of cell-intrinsic glucocorticoids signaling. Ultimately, our study has implications for the positioning of brain-body circuit for UTIs treatment. - Source: PubMed
Publication date: 2025/03/18
Liu YaxiaoWang JinhuaLin JunyangSun DingqiZhu KejiaDiao TongxiangFu QiangRen Qingyu - A series of oridonin hybrids were synthesized and evaluated for anti-inflammatory potential, focusing on their ability to inhibit NO production in RAW264.7 cells and their therapeutic prospects for NLRP3-driven disorders. - Source: PubMed
Publication date: 2025/02/27
Ou HuipingWu ZhanpanNing JinhuaHuang QiufengWang WancunYang GuochunZhou YingxunHou AnguoLi PengChen LingyunJin Wen Bin - Bioactive chitosan (Cs)-based films containing newly synthesized chitosan-furfural aminophosphonate (F) or chitosan-hydroxymethyl furfural aminophosphonate (HMF) derivatives have been investigated as potential wound dressing material. The chemical structure of the two derivatives was monitored by observing the absorption peaks of P-O-C bonds on the FTIR chart at 883 and 802 cm for F and HMF, respectively. To improve the bioavailability and bio-absorbability, F and HMF nanoemulsions were prepared and loaded separately with three different concentrations (6, 12, and 18 mL) to chitosan solution for obtaining F-loaded Cs films (CsF1, CsF2, and CsF3) and HMF-loaded Cs films (Cs-HMF1, Cs-HMF2, and Cs-HMF3). The F- and HMF-loaded Cs films were investigated via FTIR, XRD, TGA-DTG, SEM-EDX, contact angle, mechanical, and swelling behavior. CsF3 and Cs-HMF3 films (that contain highest concentration of F and HMF nanoemulsion) exhibited optimal texture properties e.g. tensile strength was 8.82 and 8.92 MPa, and swelling ratio was 53.55 and 57.7 % respectively. The films were tested against pathogenic bacteria as a model for skin-causing bacterial infection. The findings signified those films containing F or HMF exhibited larger inhibition zones than bare Cs films. The films exhibited excellent antibacterial activity against skin-infecting pathogens. In addition, Cs-HMF3 films showed exceptional antibiofilm activity against all tested bacterial strains, including multidrug-resistant pathogens. Biocompatibility assessments confirmed the safety and non-toxicity of the films, making them suitable for wound dressings. These findings highlight the potential of F and HMF-modified chitosan films as advanced wound dressing materials with enhanced mechanical properties, antibacterial efficacy, and biocompatibility, offering a promising solution for managing bacterial infections in wound care. - Source: PubMed
Publication date: 2025/03/05
El-Naggar Mehrez EAbdelgawad Abdelrahman MShaheen Tharwat IEl-Kholy Samar AHashem Mohamed MElsherbiny Dalia A - Context Infertility increases with age in various animals, including cows, owing to unknown mechanisms. The glandular and luminal epithelia of the bovine uterus synthesise and secrete colony-stimulating factor 2 (CSF2), which is a well-studied embryokine. We recently reported the possibility of fibrosis in the uteri of old cows. However, the relationship between CSF2 expression and fibrosis has not yet been clarified. Aims We tested the hypothesis that the endometrial epithelia of old cows have lower CSF2 expression compared to in heifers, and that myofibroblasts [alpha-smooth muscle actin (αSMA)-positive fibroblasts] increase near the epithelium of old cows. Methods We collected caruncle and intercaruncle samples from post-pubertal, growing, nulliparous heifers (n =6; 24.7±1.3months old) and old multiparous cows (n =6; 128.5±15.4months old). We analysed mRNA and protein expression, along with fluorescent immunohistochemistry for CSF2, anti-collagen type IV, anti-Müllerian hormone type 2 receptor, and anti-αSMA. Key results Quantitative reverse transcription polymerase chain reaction and western blot analysis revealed lower CSF2 expression in the caruncle and intercaruncle of old cows than in young heifers. Fluorescence microscopy using the same antibodies and anti-collagen type IV, anti-Müllerian hormone type 2 receptor, and anti-αSMA antibodies showed increased fibroblasts and αSMA signals near the epithelium of old cows compared to young heifers. Conclusion CSF2 expression was lower in endometrial epithelia of old cows compared to those in heifers, and myofibroblasts increased near the epithelia of old cows. Implications Lower CSF2 may play an important role in age-related infertility. - Source: PubMed
Wanjiru Denis KaraniNiyonzima Yvan BienvenuKadokawa Hiroya - Intratumoral Fusobacterium nucleatum (Fn) infection is closely associated with poor prognosis in esophageal cancer (EC) due to its impact on the tumor microenvironment (TME). The tumor cell-intrinsic cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway is critical for regulating immune cell activation in the TME. However, the link between intratumoral Fn infection and the activation of the cGAS-STING pathway in tumor cells, as well as its effects on EC progression, remains largely unknown. - Source: PubMed
Publication date: 2025/02/14
Nakajima ShotaroSaito KatsuharuFukai SatoshiSakuma MeiMatsuishi AkiraKanoda RyoMaruyama YuyaSuzuki HiroyaOkayama HirokazuSaito MotonobuMimura KosakuNirei AzumaKikuchi TomohiroHanayama HiroyukiSaze ZenichiroMomma TomoyukiNishiyama KyokoSuzutani TatsuoKono Koji