GRP78 (Bip) Antibody (OASE00405)
- Known as:
- GRP78 (Bip) Antibody (OASE00405)
- Catalog number:
- oase00405
- Product Quantity:
- USD
- Category:
- -
- Supplier:
- Aviva Systems Biology
- Gene target:
- GRP78 (Bip) Antibody (OASE00405)
Related genes to: GRP78 (Bip) Antibody (OASE00405)
- Gene:
- HSPA5 NIH gene
- Name:
- heat shock protein family A (Hsp70) member 5
- Previous symbol:
- GRP78
- Synonyms:
- BiP
- Chromosome:
- 9q33.3
- Locus Type:
- gene with protein product
- Date approved:
- 1991-07-26
- Date modifiied:
- 2015-11-19
Related products to: GRP78 (Bip) Antibody (OASE00405)
Related articles to: GRP78 (Bip) Antibody (OASE00405)
- Sleep deprivation (SD) is harmful to individuals, but its pathogenesis is not clarified. - Source: PubMed
Publication date: 2026/06/03
Gan LutongYou ZeruiTan WanyueFeng SimengCai YixianShi XianMa XiaYu JiaqiPan Jiyang - Proper proinsulin folding in the endoplasmic reticulum (ER) is prerequisite to producing bioactive insulin, and proinsulin misfolding causing β cell ER stress accompanies pancreatic β cell dysfunction in type 2 diabetes (T2D). How (and which) ER chaperones coordinate to prevent proinsulin misfolding is largely unknown other than an unspecified dependence on the hsp70 member, BiP. A genetically engineered mouse enables efficient, specific pulldown of endogenous islet β cell BiP (GRP78, the major HSP70 ER chaperone) in complexes with client proteins. We demonstrate that BiP assembles in various protein complexes (including cochaperones p58, GRP170, ERdj3, and oxidoreductases PDIA1 and PDIA6) that specifically bind to nonnative proinsulin. BiP requires p58 for productive proinsulin folding, whereas nonstoichiometric BiP excess actually hinders proinsulin folding. Coordinated and dyscoordinated BiP/cochaperone assembly in response to demand for proinsulin highlights physiologic and pathophysiologic conditions, respectively, offering a potential check point for therapeutic intervention. - Source: PubMed
Publication date: 2026/06/01
Jang InsookDuffey AlecItkin-Ansari PamelaArvan PeterKaufman Randal J - This study aimed to investigate the role of endoplasmic reticulum stress (ERS) in atopic dermatitis (AD) and its comorbid mental disorders and evaluate the therapeutic potential of mesencephalic astrocyte-derived neurotrophic factor (MANF). - Source: PubMed
Chai HengZhou JingGao YugeYang GuangyuZhang YuHao SiyuLi Yuzhen - Keratoconus (KCN) is a bilateral and asymmetric cornea disease. Autophagy plays an important role in homeostasis by protecting cells against stress. However, the roles of autophagy-related genes (ARGs) in KCN remains unclear. Hence, this study aimed to identify the signatures of ARGs of KCN and explore their correlation with immune infiltration. Transcriptional data and clinical information of patients with KCN were downloaded from the profile data GSE112155 and GSE151631. Functional analysis was used to reflect the biological functions, and weighted gene co-expression network analysis was applied to excavate co-expression modules of autophagy-related expression patterns. Moreover, gene set enrichment and variation analyses were performed for pathway analysis. Consensus clustering analysis was used to cluster different molecular subtypes on the basis of gene expression profiles of KCN-specific ARGs. Single-sample gene set enrichment analysis was employed to calculate separate enrichment scores for each immunocyte between KCN and healthy samples. Finally, hub genes were verified by real-time quantitative polymerase chain reaction. We first identified 14 ARGs differentially expressed between patients with KCN and controls using NetworkAnalyst. Nine overlapped genes (BNIP3, CDKN1A, DDIT3, FOS, HSPA5, MAPK8IP1, MYC, PPP1R15A, and VEGFA) (P < .05) were identified using a random forest model. The MAPK signaling pathway, apoptosis, FoxO signaling pathway, and protein processing in endoplasmic reticulum signaling were mainly involved. The weighted gene co-expression network analysis classified the genes into 12 distinct modules. The MEturquoise (correlation = 0.51), MEpink (correlation = 0.535) were significantly positively correlated with KCN, whereas the MEyellow (correlation = -0.776) and MEgreen (correlation = -0.664) were negatively correlated. Single-sample gene set enrichment analysis showed a close interaction between immune cell infiltration and the development of KCN. Finally, all the 9 hub genes except VEGFA were significantly downregulated (P < .05) using real-time quantitative polymerase chain reaction. We described the signatures of ARGs in KCN, the distribution of immune cells between KCN patients and the control, and the correlation between hub genes related to autophagy and KCN disease. This demonstrates the potential roles of autophagy mechanisms and the immune response in KCN, providing a novel insight into understanding the pathogenesis of KCN and potential treatment targets. - Source: PubMed
Li SutongWang GangChen JingLi Naiyang - Weaning represents a critical transition in dairy calves, involving simultaneous dietary, social, and environmental changes. This study investigated how the weaning affects immune-related gene expression in circulating leukocytes of Holstein calves and whether post-weaning growth performance is associated with distinct immunometabolic responses. Forty female calves were retrospectively classified into two groups based on average daily gain (ADG) during the 10 days following complete weaning (from 60 to 70 days of age). Calves were fully weaned at 60 days of age using a step-down approach. Animals were categorized as high ADG (HIGH, n = 20) or low ADG (LOW, n = 20) Blood samples collected at 60 and 70 days were analyzed for plasma metabolites and leukocyte transcriptomic profiles targeting 37 genes involved in innate immunity, inflammation, oxidative stress, and metabolic regulation. Weaning induced a marked activation of innate immune pathways in all calves, as evidenced by increased expression of CD14, NFKB1, IL1β, TNFα, and genes related to cell adhesion and trafficking. However, LOW calves exhibited greater transcriptional responses, with higher expression of TLR2, CASP1, IL18, CD16, and markers of oxidative (SOD2, HSPA5) at 70 days, indicating a metabolic and transcriptional profile consistent with a greater physiological burden. Additionally, at 70 days, LOW calves had lower plasma glucose, insulin, triglycerides, and cholesterol, along with higher urea and creatinine, while β-hydroxybutyrate did not differ between groups, suggesting reduced energy availability and increased reliance on amino acid catabolism. These findings suggest that calves with lower post-weaning growth exhibit greater immune activation and altered metabolic status, highlighting variability in resilience to weaning stress. Understanding such differences may support the development of targeted nutritional or management strategies to improve early-life adaptation and long-term productivity in dairy systems. - Source: PubMed
Publication date: 2026/05/27
Sfulcini MartaTrevisi ErminioPiccioli-Cappelli FiorenzoMinuti Andrea