AQP3 antibody
- Known as:
- AQP3 (anti-)
- Catalog number:
- orb96381
- Product Quantity:
- EUR
- Category:
- -
- Supplier:
- Biorbyt biorb
- Gene target:
- AQP3 antibody
Related genes to: AQP3 antibody
- Gene:
- AQP3 NIH gene
- Name:
- aquaporin 3 (Gill blood group)
- Previous symbol:
- -
- Synonyms:
- GIL
- Chromosome:
- 9p13.3
- Locus Type:
- gene with protein product
- Date approved:
- 1994-07-25
- Date modifiied:
- 2019-04-23
Related products to: AQP3 antibody
Related articles to: AQP3 antibody
- This study evaluated whether resveratrol (RESV), alone or in combination with antifreeze protein type I (AFP I), improves slow freezing cryopreservation of in vivo-derived ovine embryos. Good-quality embryos recovered non-surgically from 35 superovulated ewes were assigned to three groups: AFP (0.1 µg/mL AFP I in the freezing solution), RESV (pre-incubation for 6 h in holding medium supplemented with 1 µM RESV before cryopreservation), or RESV + AFP (combined treatment). After thawing, embryos were cultured in vitro for 48 h and assessed for several endpoints. Resveratrol exposure (RESV and RESV + AFP) reduced intracellular levels of glutathione (GSH), reactive oxygen species (ROS), and ROS/GSH ratio after incubation (P < 0.05), without affecting mitochondrial activity. After thawing, embryos in the RESV + AFP group exhibited higher mitochondrial activity than the others (P < 0.05), accompanied by increased ROS/GSH ratios compared to AFP alone (P < 0.05). After culture, redox parameters did not differ across treatments. There was no difference in survival rates or dead cell index among groups (P > 0.05), but RESV + AFP presented the highest content of intracellular lipid (P < 0.05). Finally, expression of AQP3 and PRDX1 genes was downregulated in the AFP group compared with RESV, with no difference between RESV and RESV + AFP (P > 0.05). Altogether, combining RESV with AFP I enhanced post-thaw mitochondrial activity but disrupted redox homeostasis and increased lipid accumulation, without improving embryo survival or hatching. These findings indicate that RESV combined with AFP I does not improve embryo cryotolerance, while RESV pretreatment may help reduce oxidative stress before cryopreservation. - Source: PubMed
Publication date: 2026/05/15
Guimarães Mariana P POliveira Thaís ALeal Gabriela RCupello Ana Paula SBrandão Felipe ZBatista Ribrio Ivan T PCorreia Lucas F LSouza-Fabjan Joanna M G - Zeng Ye Tang (ZYT) is used to treat constipation caused by various factors. However, the precise mechanism underlying its therapeutic effects remains unclear. - Source: PubMed
Publication date: 2026/05/14
Wu XueWan YanZhang LinaoLuo ShifangLiu FeifanBai YuanmeiLi QingTang XianjinLi TaoTang HuaZhang YanyanXie YuhuanGuo Peixin - Pancreatic ductal adenocarcinoma (PDAC) is the seventh leading cause of cancer-related mortality, with poor survival due to late diagnosis and ineffective therapies. Aquaporin-3 (AQP3) and AQP5 are transmembrane proteins overexpressed in PDAC, promoting tumor progression and metastasis, representing promising therapeutic targets. Here, we investigated their involvement in PDAC spheroids' growth and morphology using two human PDAC cell lines, BxPC-3 and MiaPaca-2. Treatment with AQP3 inhibitors decreased spheroids' diameter, area, and viability and altered MiaPaca-2 spheroids' circularity, suggesting reduced growth. Similarly, silencing AQP3 or AQP5 in BxPC-3 spheroids decreased spheroids' size with a more pronounced viability reduction, indicating impaired growth and cell death. This study demonstrates, for the first time, the critical roles of AQP3 and AQP5 in PDAC spheroids' development. - Source: PubMed
Publication date: 2026/05/17
Pimpão CatarinaEngrácia Diogo MSoveral GraçaMendes Filipa - Arsenic trioxide (ATO) is both a life-saving therapy for acute promyelocytic leukemia and a systemic toxicant whose hepatic effects remain incompletely defined. This study examined how a single clinically relevant ATO dose (8 mg/kg, i.p.) acutely remodels hepatic xenobiotic-metabolizing enzymes, arsenic transporters, and pro-inflammatory mediators in male and female C57Bl/6 mice. Mice were treated with ATO or saline and livers were collected at 6 and 24 h for integrated mRNA and protein profiling of major Cytochrome P450 (CYP) families, aquaglyceroporins (), ATP-binding cassette () transporters, and cytokines (). ATO induced highly sex-, time-, and isoform-specific reprogramming. Females exhibited a wider and earlier decline in several female-predominant CYP2, CYP3, and CYP4 isoforms, including a more pronounced reduction in hepatic CYP3A, CYP4A, and CYP4F protein abundance. In contrast, males showed mainly transcriptional induction of specific genes (, , , and ), accompanied by comparatively modest decreases in overall CYP protein levels. and transporters were differentially modulated, with males displaying early, relatively monotonic upregulation of efflux systems, while females exhibited higher basal expression but more complex, biphasic regulation of both influx () and efflux pathways. These transcriptional changes paralleled a transient inflammatory response, including early induction and female-specific elevation. Collectively, these findings highlight sex-dependent modulation of hepatic ATO handling and drug metabolizing capacity, with important implications for risk assessment and individualized ATO containing regimens. - Source: PubMed
Publication date: 2026/05/08
El-Mahrouk Sara REl-Kadi Ayman O S - Injectable sodium hyaluronate (NaHA) is extensively utilized in aesthetic medicine as a dermal hydrating agent. However, there are few standardized, human-relevant preclinical methods that can reliably evaluate the moisturizing performance of those products. Existing animal and simplified cell-based models show restricted physiological relevance and insufficient sensitivity. To address this gap, we established a depot-mimicking reconstructed human full-thickness skin (RhFS) platform incorporating an inclusion-based intradermal delivery strategy. Instead of conventional mixing, this strategy mimics the clinical 'depot effect' of intradermal injection, allowing for the simultaneous assessment of cellular responses and tissue-level hydration dynamics. It forms a localized NaHA depot within the dermal compartment of RhFS and preserves spatial hydration gradients, which are lost when NaHA is mixed homogenously. The platform integrates physicochemical characterization of polymer-bound water states with cellular and tissue-level functional readouts. By quantifying key biomarkers, including CD44, aquaporin-3 (AQP3) and natural moisturizing factors (NMFs), our results demonstrate that the inclusion-based delivery strategy significantly outperforms conventional mixing approaches in activating epidermal hydration pathways. Crucially, this platform effectively distinguished the moisturizing efficacy of multiple commercial NaHA formulas, thereby revealing a structure-activity relationship between water-binding states and biological outcomes. Overall, this study presents a reproducible, mechanism-informed and human-relevant framework for preclinical performance evaluation of NaHA-based injectable biomaterials and provides a sensitive alternative to conventional animal-based approaches. - Source: PubMed
Publication date: 2026/02/09
Shi JianfengXu WennaLiu HongfuShan FengjuanWang RuiKe LinnanHan QianqianLu Yong