Ask about this productRelated genes to: PPP3R2 antibody
- Gene:
- PPP3R2 NIH gene
- Name:
- protein phosphatase 3 regulatory subunit B, beta
- Previous symbol:
- -
- Synonyms:
- PPP3RL
- Chromosome:
- 9q31.1
- Locus Type:
- gene with protein product
- Date approved:
- 1993-01-25
- Date modifiied:
- 2016-10-05
Related products to: PPP3R2 antibody
Related articles to: PPP3R2 antibody
- Chronic stress significantly impacts hippocampal function through transcriptional and epigenetic mechanisms. While the roles of lncRNAs in stress-related transcriptional and epigenetic regulation have recently been recognized, their genome-wide functions controlling the transcriptional network remain largely unclear. Evidence indicates that the lncRNA uc.104 is involved in stress responses; however, its genome-wide chromatin interactions and gene regulatory effects are yet to be explored. To examine this, we combined chromatin isolation by RNA purification sequencing (ChIRP-seq) and RNA sequencing (RNA-seq) in the hippocampus from handled control and chronic restraint stress (CRS) rats. ChIRP-seq identified 6,664 uc.104 binding peaks under CRS, including 6,517 enriched and 149 reduced. Many peaks were mapped to intronic and promoter-proximal regions of protein-coding genes. Integration of ChIRP-seq with RNA-seq data revealed 1,839 differentially expressed genes associated with uc.104 binding sites, with 106 high-confidence overlaps. Several genes (Gabra3, Htr7, Irs1, Gpr37, Clu, Hspa1b, Ppp3r2, Nfasc, Pcdhac2, and Cysltr2) identified as regulatory targets of uc.104, have been directly implicated in stress responses, synaptic plasticity, and neuroinflammation. Gene ontology and Synapse GO (SynGO) analyses revealed significant enrichment for processes involving dendritic spine formation, synapse organization, and pre- and postsynaptic signaling. Protein-protein interaction analysis identified hub genes, including EGFR, CDC42, IGF1R, CTNNB1, CALM1, CALM3, POLR2A, MDM2, TBP, and CSNK1E, several of which have been linked to stress-responsive pathways. Together, our findings reveal that uc.104 binding to chromatin near stress- and synapse-related genes may act as a regulator of stress-responsive transcriptional networks in the hippocampus. By linking uc.104 occupancy to stress and synaptic responsive genes, this study highlights uc.104 as a potential mediator of stress-induced hippocampal malfunctions. - Source: PubMed
Publication date: 2026/04/19
Verma Anuj KRoy BhaskarPrall KevinHulwi EllieDwivedi Yogesh - Impaired sperm motility is a leading cause of male infertility. Studies indicated that FK506, an immunosuppressive drug, resulted in male mouse infertility or an overall decline in the fertilization capacity of male renal transplant recipients. However, the underlying mechanism is not fully elucidated. Here, we reported that the disruption of FK506 binding protein 12.6 (FKBP12.6) significantly alleviated FK506-induced male infertility in mice by restoring sperm motility and mitochondrial functions in immature sperm. Mechanically, we identified that the FK506-FKBP12.6 complex preferentially bound to the sperm-specific calcineurin, which is composed of a catalytic subunit (PPP3CC) and a regulatory subunit (PPP3R2). We revealed that FKBP12.6 deficiency reversed FK506-induced the elevated expression of Down syndrome critical region 1.1 (DSCR1.1, a calcineurin inhibitor) and the reduced expressions of PPP3CC and PPP3R2 in immature sperm. Additionally, we observed that FKBP12.6 deficiency remarkably improved FK506-induced the abnormality of Ca release through restoring calcineurin-mediated dephosphorylation at S2808 and S2814 of RyR2, and maintained the mitochondrial homeostasis by suppressing the hyperphosphorylation at S637 of the mitochondrial dynamin-related protein 1 (Drp1) in immature sperm. Furthermore, we demonstrated that FKBP12.6 deficiency reversed FK506-induced the reduction of the acrosome reaction in sperm by retaining the expression of synaptosome-associated protein of 25 kDa (Snap25) in immature sperm, which is essential for the maturation of acrosome exocytosis function. Certainly, our findings should provide an insight in elucidating the mechanism of FK506-induced male infertility, suggesting that FKBP12.6 might be a potential target for male infertility clinically. - Source: PubMed
Publication date: 2025/12/22
Xiao Yun-FeiYang Shi-FenHuang Shi-AngZeng Zhi-XiongGong Li-NaXie LinWang Ling-FangGuan Xiao-HuiJiang Mei-XiuQian Yi-SongDeng Ke-YuXin Hong-Bo - Samphire (), a halophyte, thrives in saline environments due to its salt tolerance, which is partly attributed to miR167. However, the functional role of miR167 in human cells is unclear. This study explores the role of extracellular vesicles (EVs) derived from C. callus in skin regeneration, highlighting the potential of miRNA tae-miR167c-5p (miR167). Calluses were successfully induced and scaled for EV isolation. Characterization confirmed the presence of plant EV biomarkers and EVs with an average size of 136.6 nm. Cm-callus EVs enhanced wound healing and skin regeneration in human fibroblasts (HFF cells and CCD-986Sk cells) by modulating key genes, in particular, by downregulating and upregulating and . Small RNA sequencing revealed an enrichment of miR167 in Cm-callus EVs. Transfection with an miR167 mimic replicated these regenerative effects. Computational predictions identified , which is linked to the MAPK and NFAT pathways, as a potential target of miR167. This study demonstrates the efficacy of Cm-callus EVs and miR167 in promoting skin regeneration without cytotoxicity, providing insights into their therapeutic potential and calling for further experimental validation of target interactions. - Source: PubMed
Publication date: 2025/08/12
Jin SollKu ChangHoeKim Hye JinKim Jae-GooKim Sang HoonHan HeyjinKang Hee CheolHwang Jae SungKim Mi Jung - The genomic effects of biomechanical loading on human growth plate cartilage are unknown so far. To address this, we used rare human growth plate biopsies obtained from children undergoing epiphysiodesis and exposed them to precisely controlled mechanical loading using a microloading device. The biopsies were cultured 24 hours after mechanical loading, followed by RNA-sequencing analyses to decipher the genomic regulation. - Source: PubMed
Publication date: 2024/12/10
Zhang ZhengpeiBoggavarapu Nageswara RaoMuhr Laila Sara ArroyoGarcia-Serrango AinhoaAeppli Tim RjNava Tobia SebastianoZhao YunhanGutierrez-Farewik Elena MKulachenko ArtemSävendahl LarsZaman Farasat - Pathological angiogenesis, the abnormal or excessive generation of blood vessels, plays an important role in many diseases including cancer, diabetic retinopathy, psoriasis, and arthritis. Additionally, increasing evidence supports the close linkage between angiogenesis and inflammation. Snake venoms are a rich natural source of biologically active molecules and carry rich potential for the discovery of anti-angiogenic and anti-inflammatory modulators. Here, we isolated and purified a novel protein, ZK002, from the venom of the snake , and investigated its anti-angiogenic and anti-inflammatory activities and mechanisms. ZK002 was identified as a 30 kDa heterodimeric protein of α and β chains, which exhibited anti-angiogenic activity in various assays. Mechanistically, ZK002 inhibited activation of VEGF signaling and related mediators including eNOS, p38, LIMK, and HSP27. ZK002 also upregulated the metalloproteinase inhibitor TIMP3 and inhibited components of the VEGF-induced signaling cascade, PPP3R2 and SH2D2A. The anti-angiogenic activity of ZK002 was confirmed in multiple models. ZK002 could also inhibit the expression of pro-inflammatory cytokines, as well as inflammation in the carrageenin-induced edema rat model. Our findings highlight the potential for further development of ZK002 as a dual function therapeutic against diseases with involvement of pathogenic angiogenesis and chronic inflammation. - Source: PubMed
Publication date: 2023/09/29
Chan Brandon DowWong Wing-YanLee Magnolia Muk-LanYue Patrick Ying-KitDai XiangrongTsim Karl Wah-KeungHsiao Wen-Luan WendyLi MandyLi Xiao-YiTai William Chi-Shing