Ask about this productRelated genes to: MYLPF antibody
- Gene:
- MYLPF NIH gene
- Name:
- myosin light chain, phosphorylatable, fast skeletal muscle
- Previous symbol:
- -
- Synonyms:
- MRLC2, HUMMLC2B, MYL11
- Chromosome:
- 16p11.2
- Locus Type:
- gene with protein product
- Date approved:
- 2009-03-06
- Date modifiied:
- 2016-07-18
Related products to: MYLPF antibody
Related articles to: MYLPF antibody
- Postpartum stress urinary incontinence (SUI) significantly impacts women's quality of life, yet the underlying molecular mechanisms of effective treatments remain elusive. This study aimed to investigate the therapeutic effects and molecular pathways of electroacupuncture (EA) in a rat model of SUI. We established the SUI model using vaginal balloon dilation (VBD) and randomized subjects into model and EA-treated groups. Functional recovery was assessed by urodynamic testing, which showed that EA significantly increased leak point pressure (LPP) and maximum bladder capacity (MBC). Histopathological analysis using hematoxylin and eosin and Masson's trichrome staining revealed improved muscle architecture and a 42.3% reduction in collagen deposition, along with suppressed inflammatory responses. Quantitative proteomics identified 2,907 differentially expressed proteins (DEPs), with the STRING database highlighting motor protein pathways as central targets. Specifically, EA selectively downregulated core proteins, including MYL1, TNNI2, MYLPF, TNNT3, and TNNT1. These findings, validated by western blot and qPCR, suggest that EA alleviates SUI by modulating muscle contractility and remodeling the pelvic floor, providing insight into potential mechanisms underlying targeted SUI therapy. - Source: PubMed
Publication date: 2026/04/07
Jiang XiaoyuLiang HuilingSheng WeiweiSun Jian - Myofiber type determines meat quality by governing postmortem glycolytic potential and lactate-driven pH decline. However, whether endogenous lactate actively regulates myofiber type through epigenetic signaling remains unknown. Here, we found that histone H3 lysine 27 lactylation (H3K27la), a modification driven by lactate, is a critical regulator of myoblast differentiation. Elevating lactate enhanced differentiation and H3K27la levels in myoblasts. Multi-omics analyses revealed that H3K27la enrichment is associated with transcriptional repression of the member of RAS oncogene family (RAP2C) during goat myoblast differentiation. This inactivation of the RAP2C-ERK-ELK1 signaling axis subsequently upregulates myosin light chain, phosphorylatable, fast skeletal muscle (MYLPF), thereby promoting glycolytic myofiber specification. Our study uncovers a lactate-H3K27la regulatory axis that translates the metabolic state of developing muscle into an epigenetic signal that orchestrates myofiber type, providing profound insights into the early-life programming of myofiber determination and meat quality. - Source: PubMed
Publication date: 2026/04/20
Zhang ZhenZhou XiaohuaHuang XinaiYang YueDeng KaipingZhou JunLiu ZhipengLi JingzhengLi DongxuEl-Samahy M AZhang GuominFan YixuanWang Feng - Protected Designation of Origin (PDO) beef products are recognized for their higher quality, although the native breeds used often show lower productivity compared to commercial crosses. This study aimed to compare the proteome profiles of Arouquesa and crossbred beef using 2D-DIGE, and evaluating crude protein, energy, and fatty acid composition. Arouquesa beef had higher energy values ( < 0.05), whereas crossbred samples showed greater protein content ( < 0.05). Moreover, Arouquesa beef had higher proportions of microminerals, namely zinc, iron, and manganese ( < 0.05). The Arouquesa breed also presented higher proportions of most fatty acids, including saturated, monounsaturated, and polyunsaturated. Proteomic analysis identified 34 differentially abundant proteoforms from 23 proteins. In Arouquesa, proteins associated with muscle contraction (MYLPF, TNNT1), glycolysis/gluconeogenesis (TPI1, GPD1), oxygen transport (HBB, HBA), and heat shock response (CRYAB, HSPB1) were more abundant or had altered spot patterns, suggesting their roles in meat tenderness, color, and fat-related characteristics. Conversely, crossbred beef showed elevated levels of glycolytic enzymes (PGM1, ENO3, and some proteoforms of TPI1 and GPI) and structural proteins (MYL1), related with higher muscle growth and different fiber composition. The identified proteins provide possible molecular markers that distinguish Arouquesa from commercial breeds. These validated proteins in the future can support breed characterization and verification of PDO certification specifications. - Source: PubMed
Publication date: 2025/12/19
Sacarrão-Birrento LauraSchlosser SarahHummel KarinRazzazi-Fazeli EbrahimMartins Cátia FMourato Miguel PSilva José ASilva Severiano RAlves Susana PVenâncio Carlos AMiller Ingridde Almeida André M - To investigate the protective mechanism of Astragaloside IV (AS-IV) in diabetic retinopathy(DR).A streptozotocin-induced diabetic rat model was established and divided in to three groups: control, DR, and DR + AS-IV. Retinal injury was assessed using optical coherence tomography (OCT). Retinal proteomes were profiled using 4D-DIA proteomics. Candidate genes were validated using quantitative PCR (qPCR).302 differentially expressed proteins were detected. Venn diagram analysis revealed three down-regulated proteins in the DR group: Gnal, Dennd1a, and Snx13, and two up-regulated proteins: Ogn and Mylpf. AS-IV treatment reversed the expression of Dennd1a and Gnal while downregulating Ogn, Mylpf, and Snx13. PPI analysis revealed limited direct connectivity among the five proteins but identified 10 additional interactors, including MYLK, ADCY9, and RAB35. GO analysis indicated involvement in muscle contraction, muscle myosin complex, and phosphatidylinositol phosphate binding and structural molecule activity. KEGG analysis highlighted calcium signalling as a key pathway. Molecular docking demonstrated stable interactions between AS-IV and Dennd1a, Ogn, and Snx13 proteins. qPCR confirmed significant regulation of Dennd1a and Ogn, while Snx13 and Mylpf changes were not significant.AS-IV exhibited protective effects against diabetic retinal injury by modulating Dennd1a and Ogn, implicating calcium signalling and structural pathways in its therapeutic mechanism. - Source: PubMed
Publication date: 2026/01/25
Zhang XingyiLiu Hua - Chronic kidney disease (CKD) is highly prevalent among individuals with abnormal glucose metabolism. However, limited research has specifically investigated CKD-associated proteins within this high-risk population. To address this gap, our study aimed to identify proteins associated with CKD in participants with abnormal glucose metabolism, potentially informing early detection and targeted therapeutic strategies. - Source: PubMed
Publication date: 2025/09/09
Li NingLiu JingyangWu GuanghengZhang JieLiu LongZheng ManqiLi HaibinLi ChangweiWen YaluJi JianguangYu YangZhao KunZheng Deqiang