Ask about this productRelated genes to: NIFK antibody
- Gene:
- NIFK NIH gene
- Name:
- nucleolar protein interacting with the FHA domain of MKI67
- Previous symbol:
- MKI67IP
- Synonyms:
- Nopp34, hNIFK
- Chromosome:
- 2q14.3
- Locus Type:
- gene with protein product
- Date approved:
- 2002-05-03
- Date modifiied:
- 2019-02-08
Related products to: NIFK antibody
Related articles to: NIFK antibody
- Long-term continuous rice cultivation depletes soil nutrients and disrupts microbial nitrogen cycling, whereas tobacco-rice rotation (TRR) serves as a potential strategy for restoring soil functionality. This study reveals that TRR integrates the plant secondary metabolite nicotine into the nitrogen cycle through synergistic bacterial-archaeal metabolism, forming a "nicotine-molybdenum (Mo)-nitrogen" coupled metabolic network. Nicotine is degraded into ammonia and other nitrogen-containing intermediates via cross-domain collaboration involving bacteria (e.g., Ramlibacter, Streptomyces) and archaea (e.g., Nitrososphaera, Methanopereden). This process is supported by significant upregulation of key nitrogen cycling genes in TRR soils, including nifK (nitrogen fixation), nasA (nitrate reduction), and ndhABC (nicotine degradation. Available Mo content in TRR soils is significantly reduced (0.27 ± 0.02 mg/kg compared to 0.35 ± 0.03 mg/kg in continuous rice cultivation, p < 0.05), indicating that Mo ions are a critical factor in this process. However, long-term TRR may deplete soil Mo reserves, potentially limiting nitrogen availability and highlighting the need for Mo supplementation strategies. This study proposes a "nicotine-Mo-nitrogen" theoretical model, refining the conventional understanding of soil nitrogen cycling and providing a theoretical foundation and practical insights for microbially driven soil health management in sustainable agriculture. - Source: PubMed
Publication date: 2026/05/15
Li XuanZhuang FanWu ShaolongHuang XiaohuiXiao YansongZhou XiangpingTeng KaiChen YiqiangHe WeiDeng ZhengyuHuang QiWang CanLiu ZhenghuaYin HuaqunZou JunliangMeng DelongLiu TianboLuo Kun - The extensive accumulation of coal gangue poses significant environmental threats through water contamination, soil degradation, and atmospheric pollution, necessitating the urgent development of ecological utilization strategies. This study elucidates the mechanistic basis by which the thermophilic bacterium () enhances plant growth in coal gangue-amended sandy soils. Through integrated analysis of nutrient dynamics, phytohormonal activities, soil enzymatic profiles, and metagenomic functional profiling, we demonstrate significant synergy between coal gangue and . When applied together in sandy soils, the germination rate, plant height, root length, and fresh biomass of (alfalfa) increased by 1.18-2.06 times. The levels of soil nitrogen, phosphorus, and potassium also significantly increased, resulting in notable improvements in soil fertility. The bacterial treatment enhanced the activities of indole-3-acetic acid, 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and various soil enzyme activities while also optimizing the microbial community structure and increasing the abundance of beneficial bacteria, including . Metagenomic analysis revealed the upregulation of growth-promoting genes such as , , and , which collectively drive plant growth through multiple pathways, including enhanced soil nutrient availability, hormone regulation, soil enzyme activities, and nutrient cycling. Collectively, this work deciphers molecular-scale bacteria-gangue synergism, providing a theoretical foundation for sustainable coal gangue utilization and ecological restoration of degraded soils. - Source: PubMed
Publication date: 2026/04/24
Liu MingwuDu MeiliXi ZijieTastambek Kuanysh TBao YuanSong XiaonanZhou AnningWang Yaya - Bacterial ribonucleases (RNases) are central components of post-transcriptional networks underlying environmental adaptation. However, their contribution to the ecological specialization of bacteria with complex lifestyles, such as nitrogen-fixing legume symbionts, remains poorly understood. Here, we investigated the role of the double-stranded RNase III ortholog (SmRNase III) in Sinorhizobium meliloti, the symbiotic partner of alfalfa (Medicago sativa L.). Loss of SmRNase III function affected the expression of nearly 30% of protein-coding genes and 12% of annotated non-coding RNAs (sRNAs). Remarkably, more than 70% of these changes occurred under the microaerobic conditions typical of symbiotic nodules. Many SmRNase III-dependent transcripts encode pathways supporting microaerobic metabolism and nitrogen fixation in endosymbiotic bacteroids. Analysis of sequencing read coverage identified putative consensus cleavage signatures enriched in mRNA 5' untranslated regions, suggesting preferential processing at these sites. Altered expression of sRNAs and/or their predicted mRNA targets further supports a role for SmRNase III in sRNA-mediated silencing. Consistently, in vitro assays showed that base‑pairing between nifK (encoding the β‑subunit of the nitrogenase MoFe protein) and the antisense RNA asNifK promotes SmRNase III-mediated cleavage. In vivo assays further supported that silencing of nifK and dctA (encoding a major dicarboxylate transporter) requires SmRNase III, with dctA regulation involving a base‑pairing interaction between the trans‑sRNA AbcR1 and a predicted SmRNase III cleavage site within the mRNA. Our findings reveal a major impact of SmRNase III on shaping the symbiotic transcriptome of S. meliloti and provide a foundation for deeper investigation into RNase III-mediated regulation in rhizobia. - Source: PubMed
Publication date: 2026/04/15
Guedes-García Sabina KGarcía-Tomsig Natalia IMatos Rute GSaramago MargaridaArraiano Cecilia MJiménez Zurdo José I - - Source: PubMed
Publication date: 2026/03/14
Zhou YiHuang LinaYang HongyuWang ShupingWang FeiLi LiHuang Guowei - N-fixing bacteria have great potential to be used as biofertilizer in agriculture to promote plant growth via nitrogen fixation. In this study, a novel species sp. nov., with strain BL-9 as the type strain, was isolated from the rhizosphere of . Strain BL-9 was able to fix nitrogen and grow on nitrogen-free medium. Phylogenetic analysis of 16S rRNA gene revealed that strain BL-9 was most closely related to BAPVE7B (98.03%), followed by A4STR04 (96.72%), (96.6%), (96.6%), and DB13260 (96.57%). The phylogenomic tree supported that strain BL-9 was most closely related to BAPVE7B. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) between strain BL-9 and its closely related type strain, BAPVE7B, were 42.5% and 90.94%, respectively, which were below the values (70% for dDDH and 95% for ANI) for species discrimination. The DNA G+C content of strain BL-9 was 49.7%. The genome of strain BL-9 had a (trogen ixation) gene cluster containing 10 genes (). The predominant fatty acid was anteiso-C15:0, the major menaquinone was MK-7, and the major polar lipid was diphosphatidylglycerol. Strain BL-9 and its closely related species of had some common and distinguished physiological characteristics. Based on genomic, phylogenetic, chemotaxonomic, and phenotypic features, strain BL-9 represents a novel species of the genus The name proposed for this species is sp. nov., with the type strain BL-9. - Source: PubMed
Publication date: 2025/12/25
Hu RuiShang YiminZhang WeilongSong ChengaoWang RenzongChen Sanfeng