Ask about this productRelated genes to: ZDHHC3 antibody
- Gene:
- ZDHHC3 NIH gene
- Name:
- zinc finger DHHC-type containing 3
- Previous symbol:
- -
- Synonyms:
- ZNF373, GODZ, DHHC3
- Chromosome:
- 3p21.31
- Locus Type:
- gene with protein product
- Date approved:
- 2002-04-04
- Date modifiied:
- 2017-02-20
Related products to: ZDHHC3 antibody
Related articles to: ZDHHC3 antibody
- ARL15 is a member of the ARF-like (ARL) family of small GTPases, implicated in the regulation of ion homeostasis and metabolic signaling pathways. Although ARL15 has been suggested to undergo S-acylation-a reversible lipid modification that governs membrane association and trafficking-the stoichiometry of this modification and the responsible S-acyltransferases have remained unclear. Here, we systematically characterized the S-acylation of ARL15 and identified the enzymes mediating this modification. Using acyl-PEGyl exchange gel-shift assays, we show that ARL15 is triply S-acylated at three conserved N-terminal cysteine residues (Cys17, Cys22, and Cys23) in HEK293T cells. Single cysteine-to-serine mutations substantially reduced S-acylation, whereas substitution of all three cysteines abolished it entirely. Loss of S-acylation disrupted membrane association of ARL15, as shown by confocal imaging and subcellular fractionation. A candidate screen using siRNA knockdown and CRISPR/Cas9-mediated gene disruption revealed that the Golgi-localized S-acyltransferases ZDHHC7 and ZDHHC3 mediate ARL15 S-acylation in a partially redundant or parallel manner. Dual inhibition of both enzymes led to a marked reduction in S-acylation and redistributed ARL15 from membranes to the cytosol. These findings elucidate the stoichiometry and enzymatic regulation of ARL15 S-acylation and provide mechanistic insight into its subcellular localization. - Source: PubMed
Publication date: 2026/04/16
Chino TakeshiAraki MakotoAshi YukiIzawa YusukeNunami YujiIchikawa YosukeKuroiwa ItsukiKontani Kenji - Protein palmitoylation, a key posttranslational modification, is involved in cell signaling, protein stability, and tumor immune microenvironment (TIME) regulation. ZDHHC3 is known to mediate clear cell renal cell carcinoma (ccRCC) immune evasion by enhancing PD-L1 stability, but the roles of other palmitoyltransferases in ccRCC remain unclear. In this study, we analyzed genomic and transcriptomic data via a multiomics approach to investigate the expression patterns and potential regulatory mechanisms of known palmitoylation enzymes in ccRCC. - Source: PubMed
Publication date: 2026/03/06
Guo ABaoLu YaoWang JunJia ZhanKuiYang JinJianNing Xianghui - Intracranial aneurysms (IAs) can cause life-threatening subarachnoid hemorrhage upon rupture, yet their molecular mechanisms remain poorly understood. Proteins encoded by the ZDHHC genes mediate protein palmitoylation and are involved in brain injury processes, but their relationship with IA rupture is unclear. - Source: PubMed
Publication date: 2026/02/19
Zhang WanjingLiu LuHou YajunWang Weiqi - This study investigated the relationship between the ketogenic diet, RGMa methylation, and GluA1 palmitoylation in a rat model of epilepsy, aiming to identify a novel mechanism for treating refractory epilepsy with the ketogenic diet. - Source: PubMed
Publication date: 2026/02/05
Xu JianingHuo LiangLiu JiatongXu WeiYu Tao - Rheumatoid arthritis-associated interstitial lung disease (RA-ILD) is a major cause of mortality among patients with rheumatoid arthritis (RA), yet the pathological mechanism linking joint and lung involvement remains poorly understood. The pathogenic role of Porphyromonas gingivalis (P.g) in RA has been established. This study investigated how P.g infection drives RA-ILD through palmitoylation-mediated epithelial-myofibroblast transition (EMyT), thereby uncovering a mechanistic basis for the oral-joint-lung connection. - Source: PubMed
Publication date: 2026/01/16
Li ChunfengLiu ZhipuZhao NaPan HongweiWang HongxingZhang Yi