Ask about this productRelated genes to: DNAJB11 antibody
- Gene:
- DNAJB11 NIH gene
- Name:
- DnaJ heat shock protein family (Hsp40) member B11
- Previous symbol:
- -
- Synonyms:
- EDJ, HEDJ, ERdj3
- Chromosome:
- 3q27.3
- Locus Type:
- gene with protein product
- Date approved:
- 2001-03-09
- Date modifiied:
- 2016-10-05
Related products to: DNAJB11 antibody
Related articles to: DNAJB11 antibody
- BackgroundPhysical exercise shows neuroprotective and anti-inflammatory effects in Alzheimer's disease (AD) models, but whether it modulates neuroinflammation through regulation of peripheral T-cell activity is still unresolved.ObjectiveThe present study aimed to explore the mechanisms by which aerobic exercise regulates peripheral T cell-mediated immune responses and their potential contribution to neuroinflammation in AD.MethodsMale wild-type mice and APP/PS1 transgenic mice were divided into four groups: wild-type sedentary mice (WT-SE) group, wild-type exercise group (WT-EX), APP/PS1 transgenic AD sedentary mice (AD-SE) group, and APP/PS1 transgenic AD exercise mice (AD-EX) group. The sedentary groups received no exercise training, while the exercise groups underwent a 3-month treadmill aerobic exercise intervention. At the end of the intervention, T lymphocytes were isolated from spleens. Label-free proteomics combined with LC-MS/MS was used to identify differentially expressed proteins (DEPs) and perform functional and pathway enrichment analyses. Differentially expressed protein-coding genes were validated at the mRNA level using RT-qPCR.ResultsA total of 3399 proteins were quantified across the four groups. Applying a threshold of |log fold change| > 0.67 and < 0.05, 913 DEPs were identified. These DEPs were significantly enriched in biological processes including immune system processes, protein-containing complexes, and structural molecule activity, as well as signaling pathways including AD, TGF-β, and apoptosis.ConclusionsOverall, HSP90AB1, HSP90AA1, BAG5, DNAJC8, CTSD, and ANXA1 may play a role in peripheral T-cell immune dysregulation in AD, with potential implications for central neuroinflammation. Furthermore, the beneficial effects of aerobic exercise on AD-related peripheral immune alterations, and its potential modulation of neuroinflammation, may be associated with expression changes in DEPs including Ppp2r1b, Pde2a, Casp8, Apaf-1, Dnajb11, and Dnajc13. - Source: PubMed
Publication date: 2026/03/30
Ye XingHu KaiLiu Renyi - The heat shock response (HSR) is a conserved cellular mechanism that safeguards cells against various stressors by inducing heat shock proteins (HSPs). However, to date, no study has employed the HSR to develop a prognostic risk model aimed at predicting the outcomes and directing the treatment strategies for hepatocellular carcinoma (HCC) patients. In this study, we identified two distinct molecular subgroups of HCC patients based on the expression of 37 key HSR-related genes (HRGs). These subgroups exhibited significant differences in immune infiltration, drug responsiveness, and immunotherapy efficacy. Notably, cluster 1 (C1) patients showed greater sensitivity to chemotherapy, while C2 patients were more responsive to immunotherapy. Six core HRGs (CD4, CDK5, CDKN2A, DNAJB11, HBB and TRPV4) were identified as potential biomarkers through machine learning algorithms. A risk score model incorporating these HRGs was developed to predict HCC prognosis. The expression of these HRGs was validated using immunohistochemistry (IHC), single-cell RNA sequencing, and spatial transcriptomics. Importantly, core HRGs were significantly correlated with ferroptosis, and RSL3 treatment markedly affected the expression levels of these HRGs in HCC cells. Moreover, knockdown of DNAJB11 significantly suppressed cell growth, inhibited migratory and invasive capacities, and enhanced RSL3-induced ferroptosis. This study provides the first comprehensive analysis of HSR in HCC, offering a novel molecular classification and prognostic tool to guide personalized treatment strategies. - Source: PubMed
Publication date: 2026/02/13
Geng WenhanLi TiantianZhang JinglinShen MuzhengZhang JinruiLi JinghanMa XiaojieChen JianSun HaojinLiu XiaopengCao PengxiuFan YumeiTan Ke - The endoplasmic reticulum stress (ERS) plays an important role in lipid deposition in mammals, but little is known in bird. In the present study, forty-two pairs of adult pigeons were randomly divided into the following 7 groups: Day 4 (I4), Day 10 (I10), and Day 17 (I17) of incubation, Day 1 (R1), Day 7 (R7), Day 15 (R15), and Day 25 (R25) of chick rearing. Crop tissues were sampled, and Gene Ontology (GO) enrichment analysis revealed upregulated differentially expressed genes were enriched in ERS, IRE1/PERK/ATF6-mediated unfolded protein response (UPR), and lipid synthesis-related pathways (I4 vs. R1). Additionally, accumulation of lipid droplets was greater at R1. The transcript levels of HSPA5 in males and females were upregulated at R1. The proteins upregulated at I17 and R1 in male and female pigeons included XBP1s, PERK, p-eIF2α, and ATF6. The mRNA abundances of the lipogenic genes of ACLY, ACACA, FASN, ELOVL6, GK, and DGAT2 showed distinct sex-specific timing, with peaks occurring from I17 to R1 in males and peaks occurring from R1 to R7 in females. Eighteen pairs of adult pigeons at I14 were assigned to 3 groups, namely, the C, T1 and T2 groups; pigeons in these groups were fed 4-PBA for one week at dosages of 0, 50 mg, and 100 mg (per pigeon/day), respectively. Oil Red O staining revealed 4-PBA attenuated lipid accumulation in pigeons. Treatment with 4-PBA significantly downregulated transcripts of HSPA5, ATF3, and DNAJB11 in males and HSPA5, DNAJB11, and BHLHA15 in females. Moreover, 4-PBA significantly decreased the translational levels of XBP1s, PERK, p-eIF2α, and ATF6 in males and XBP1s and PERK in females. Moreover, the transcript levels of ACACA, FASN, ELOVL6, and DGAT2 in males and those of ACLY and DGAT2 in females were also inhibited by 4-PBA. In conclusion, ERS-mediated UPR contributes to and is functionally linked with the elevated lipid synthesis (upregulated genes like ACLY, FASN, DGAT2, etc) during pigeon milk production. - Source: PubMed
Publication date: 2026/01/11
Wang LiuxiongChen JiayiMeng XinmingZheng MingdeShi YongqiZhang ZiyuZhu JianguoGong DaoqingXie Peng - Autosomal dominant polycystic kidney disease (ADPKD) is the most common inherited kidney disorder and a leading cause of kidney failure worldwide. Disease progression is driven by cyst expansion, tubular injury, and maladaptive metabolic and hemodynamic changes. Tolvaptan remains the only US Food and Drug Administration-approved disease-modifying therapy, however its tolerability and safety profile highlight the need for additional strategies. Sodium-glucose cotransporter 2 inhibitors (SGLT2is), initially developed for glycemic control, have demonstrated robust kidney-protective and cardioprotective effects across diverse patient populations, including those without diabetes. By lowering intraglomerular pressure, metabolic reprogramming, and attenuating hypoxic microenvironment and inflammation, SGLT2is target mechanisms that are relevant to ADPKD. However, concerns that SGLT2i may provoke osmotic diuresis and activate vasopressin led to systematic exclusion of patients with ADPKD from pivotal outcome trials. The 2025 Kidney Disease Improving Global Outcomes ADPKD guidelines explicitly advise against the use of SGLT2i for slowing kidney function decline in ADPKD. In this review, we examine mechanistic intersections between SGLT2i and ADPKD pathophysiology, summarize available preclinical and early clinical data, and discuss ongoing large trials designed to address safety and efficacy of SGLT2i in patients with ADPKD. We also highlight the importance of genetic heterogeneity, as most ongoing trials are enriched for rapid progressors carrying PKD1 or PKD2 pathogenic variants, while other genotypes such as DNAJB11 , ALG8 , and ALG9 , often associated with greater interstitial fibrosis, are underrepresented. Substratification of patients by genotype, risk of progression, and comorbidities will be essential to guide precision application of SGLT2i. Whether SGLT2i can ultimately be positioned as an adjunctive or standalone therapy for ADPKD remains unresolved. - Source: PubMed
Publication date: 2025/11/10
Bou Antoun Marie ThereseBorghol Abdul HamidSouvalian LevonHadla MohamadAbboud GeorgesGhanem AhmadMunairdjy Debeh Fadi GeorgeMardirossian Jean MarcSalih MahdiGarimella Pranav SVallon VolkerChebib Fouad T - Thermal stress poses a significant challenge to fish physiology and aquaculture sustainability, necessitating an in-depth understanding of molecular responses to temperature fluctuations. This study characterizes the Hsp40 gene family in Hong Kong catfish (Clarias fuscus), a species known for its thermal resilience, to elucidate their roles in proteostasis and immune modulation. A total of 55 Hsp40 genes were identified and categorized into three subfamilies: DnaJa (n = 6), DnaJb (n = 16), and DnaJc (n = 33). These genes exhibit conserved J-domains and diverse motifs, indicating adaptations to aquatic stressors. Phylogenetic analysis reveals that the Hsp40 gene family in C. fuscus exhibits the closest evolutionary relationship to Ictalurus punctatus, followed by Danio rerio, with distinct clustering patterns across the subtypes. Collinearity and synteny analyses indicated substantial genomic conservation of Hsp40 genes between C. fuscus, I. punctatus, and D. rerio, with evidence of gene duplication and homologous relationships. The subcellular localization of Hsp40 proteins was observed in the cytoplasm, endoplasmic reticulum, mitochondria, nucleus, and extracellular regions, supporting their roles in chaperone activity and immune response regulation. Protein interaction networks identified two major clusters: one associated with protein folding and another with Hsp90-dependent immune signaling. Molecular docking confirmed stable interactions between Hsp40 and Hsp70, aiding in the mitigation of thermal-induced protein misfolding. Transcriptomic analysis under thermal stress showed significant differential expression of 30 Hsp40 genes, with dnajb11 and dnajc5.1 notably differentially expressed in the liver, gills, and kidneys. Under normal-temperature conditions, 10, 6, and 12 genes were differentially expressed in the liver, gills, and kidneys, respectively. High-temperature acclimation significantly increased the number of differentially expressed genes in the kidneys (20 genes), indicating enhanced thermal tolerance. These genes bolster immunity by stabilizing proteins and modulating inflammatory pathways. These findings suggest that key Hsp40s, such as dnajb11 and dnajc5.1, serve as biomarkers for selective breeding and support the development of strategies like immunomodulatory feed additives to enhance thermal resilience in C. fuscus aquaculture. - Source: PubMed
Publication date: 2025/10/21
Ye MinghuiZhou DayanKong LingweiZhang YuleiPan ChuanhaoHuang CailinLi GuangliChen HuapuTian Changxu