Ask about this productRelated genes to: Nanog protein
- Gene:
- NANOG NIH gene
- Name:
- Nanog homeobox
- Previous symbol:
- -
- Synonyms:
- FLJ12581, FLJ40451
- Chromosome:
- 12p13.31
- Locus Type:
- gene with protein product
- Date approved:
- 2003-09-10
- Date modifiied:
- 2014-11-19
Related products to: Nanog protein
Related articles to: Nanog protein
- High‑throughput transcriptomic technologies offer a systems‑level approach to unravel the mechanisms of complex immune disorders. Inflammatory Bowel Disease (IBD), a classic example of such disorders, involves intricate interactions between genetics, microbiota and immune dysfunction. The nuclear receptor farnesoid X receptor (FXR) is implicated in IBD, but its precise mechanisms remain unclear. To investigate the role of FXR in ulcerative colitis (UC), the present study employed an integrative transcriptomic strategy, combining bulk transcriptomics (GSE75214, GSE13367 and GSE87466) and single‑cell RNA‑sequencing data (GSE116222) of human UC samples obtained from the Gene Expression Omnibus database. Subsequently, these findings were validated in a dextran sulfate sodium‑induced colitis model using FXR global knockout mice. The results revealed that FXR expression is downregulated in UC and co‑localizes with the stem cell marker CD133 in intestinal crypts. FXR deficiency exacerbated dextran sulfate sodium‑induced colitis, impaired the expression of stemness‑associated transcription factors (octamer‑binding transcription factor 3/4, homeobox protein NANOG, transcription factor SOX2 and Sal‑like protein 4), and activated the NF‑κB pathway, leading to increased production of pro‑inflammatory cytokines, specifically TNF‑α and IL‑1β. By integrating bulk and single‑cell transcriptomics with genetic validation, the present study uncovered an FXR‑dependent mechanism linking intestinal stem cell dysfunction to NF‑κB‑driven inflammation in colitis, and established a generalizable multi‑layer transcriptomic dissection strategy for complex inflammatory disorders. - Source: PubMed
Publication date: 2026/05/29
Lin HaoCheng XinranRen YangyangZhang YongmeiChen WeixuLu QingyanTian Yiqing - Triple-negative breast cancer (TNBC) is an aggressive subtype with limited targeted treatment options, making it difficult to manage effectively. This study investigates the anticancer potential of eco-friendly zinc oxide nanoparticles (SO-ZnO NPs), synthesized using Saccharum officinarum (jaggery) extract, against MDA-MB-231 breast cancer cells. The NPs were characterized by various techniques, confirming their spherical morphology, a primary particle size of 20-50 nm, aggregation into larger clusters (100-250 nm), and a moderately positive surface charge. The composition was predominantly zinc (96.1%), oxygen (2.3%), and carbon (1.59%), attributed to bioorganic capping agents. SO-ZnO NPs showed significant anticancer effects, with an IC50 of 125.00 ± 2.60 µg/mL, indicating potent cytotoxicity above this concentration. They also suppressed key inflammatory markers (IL-6 secretion and mRNA expression) and inhibited the proliferation of cancer stem cells. Notably, SO-ZnO NPs downregulated stemness markers (NANOG, OCT4, CD44) and disrupted mammosphere formation, suggesting a potential to target cancer stem cell self-renewal and modulate the tumor microenvironment. These findings underline the therapeutic potential of SO-ZnO NPs in treating TNBC, though further in vivo studies are necessary to evaluate their safety and clinical effectiveness. - Source: PubMed
Publication date: 2026/05/28
Farooqi Muhammad AwaisKim Kyeoung CheolKim Ji-HyangShamamedov DovranFarooqi Hafiz Muhammad UmerKang Chul Ung - Narghile (waterpipe) tobacco use has increased worldwide, partly due to limited awareness of its harmful effects. Despite the widespread misconception that the water filters toxic substances, the chemical composition of narghile smoke is comparable to that of cigarette smoke. However, its effects on airway tissues remain poorly understood. - Source: PubMed
Publication date: 2026/05/25
Pilati Sarah Freygang MendesDaniel Filipe IvanPilati Paulo Vinicius FontanellaMarins Mariana HornungRodrigues Aline Cristina BatistaModolo Filipe - Germline stem cells (GSCs) are crucial for gametogenesis, genetic conservation, and molecular breeding. Although GSCs lines have been well studied in mammals and several model teleost species, progress in commercial marine teleosts remains limited. In this study, we report a successful establishment of a long-term stable ovarian cell line derived from the Asian seabass (), designated the Seabass Ovarian (SBO) cell line. Ovaries were dissociated using a combined collagenase-trypsin digestion protocol; the cells were propagated and maintained in DMEM supplemented with bFGF, LIF, and fish serum. The SBO cells exhibited strong alkaline phosphatase activity. Furthermore, the cultured cells robustly expressed both germ-cell specific markers (Vasa) and pluripotency associated proteins (Nanog, SSEA-1). These findings indicate the successful isolation and long-term maintenance of an ovarian cell line expressing female germline stem cell markers from Asian seabass ovaries. The established cell line not only provides a valuable in vitro model for elucidating the mechanisms behind germ cell differentiation but also serves as a crucial cellular resource for advancing genetic breeding, germplasm preservation, and surrogate broodstock technologies in marine teleosts. - Source: PubMed
Publication date: 2026/05/21
Zhang RuobingZhan ZeyuZhao MinglianLi YiyingXu Hongyan - A central question in molecular genetics concerns how transcriptional regulatory sequences and de novo genes originate and reach evolutionary fixation. In this study, we utilize the human bicistronic gene as a model to analyze the evolutionary trajectories of gene development. This locus comprises several functional units: three enhancers (one featuring an embedded silencer), an exonic silencer that partially overlaps an ORF, a highly conserved ancestral sequence encoding a 68 aa microprotein, and a human-specific de novo gene encoding a 107 aa protein expressed spatiotemporally in embryonic brain tissues. The alignment of gene sequences from different species was used to determine the evolutionary development of enhancers and silencers, and the development of the exonic silencer was determined through application of the cultivator model and assessment of nearest-neighbor bases. : We identify significant disparities in formation mechanisms; for example, the NANOG hESC enhancer originated simply via two insertions that constitute the enhancer. In contrast, the exonic silencer (a segment of the ATAC-STARR-seq lymphoblastoid silent region 13815)-a distinct, novel type of silencer-originated from a combination of diverse mechanisms, including a "cultivator gene" process of base pair fixation, consistent with the cultivator model proposed by Li Zhao and coworkers. exemplifies novel development mechanisms occurring over hundreds of millions of years, culminating in the birth of a human-specific, de novo 107 aa cistron. The associated complex of enhancers and silencers suggests intricate regulation of the 107 aa protein in fetal brain tissues. - Source: PubMed
Publication date: 2026/04/28
Delihas Nicholas